Updated project metadata. We have performed an unbiased, open-search analysis of the oxidative PTMs and quantitative multiplexed proteomics that take place in the mouse heart tissue during the first 48h after I/R. Surgically-induced MI was performed and animals were sacrificed at 10 min, 20 min, 40 min, 80 min, 2 hours, 6 hours, 12 hours, 24 hours, and 48 hours after reperfusion (n = 3 per group), and 3 mice were sacrificed with no intervention and used as control group. Whole hearts tissue was collected for proteomics evaluation.