Updated project metadata. Nicotiana benthamiana tissue expressing either AvrRpm1-3xFlag, StrepII-3xHA-MAMI, or co-expressing AvrRpm1-3xFlag and StrepII-3xHA-MAMI were subjected to enrichment of ADP-ribosylated proteins using the engineered Af1521 Macro domain (Nowak et al., https://doi.org/10.1038/s41467-020-18981-w). A non-functional Af1521 G42E mutant of the engineered Macro domain was used as control. Enriched proteins were analyzed by LC-MS/MS.