In this project, Label-free quantitative proteomics technology was used to carry out research. A total of 2615 proteins were identified in 6 samples, of which 2515 were quantitative proteins. The significantly differentially expressed proteins were screened according to the criteria of expression change of more than 1.5 times (up-regulated greater than 1.5 times or down-regulated less than 0.67 times) and P value< 0.05. Taking the B.V.A comparison group as an example, the expression levels of 85 proteins were significantly changed, 36 proteins were significantly up-regulated, and 49 proteins were significantly down-regulated. Based on the above data, all the identified proteins were systematically analyzed by bioinformatics, and all the differentially expressed proteins were analyzed by functional enrichment and protein interaction network