SILAC-based mass spectrometry analysis for identification of eIF4A1-bound proteins in HEK293 cell line. Lysates from naive cells (light-isotope labeled) or cells expressing SBP-tagged eIF4A1 (heavy-isotope labeled) were subjected to streptavidin pulldown. Isolated proteins were pooled and subject to liquid chromatography-tandem mass spectrometry (LC-MS/MS).