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PXD045012 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAutomating data analysis for hydrogen/deuterium exchange mass spectrometry using data-independent acquisition methodology
DescriptionWe developed an HX-MS2 strategy that supports the acquisition of CID-generated fragment ions alongside their peptide precursors. The approach enables true auto-validation of HX data by mining the rich set of deuterated fragments, using the extra dimension of data to simultaneously confirm the peptide ID and authenticate (even correct) MS1-based deuteration calculations. The high redundancy provided by the fragments generates an opportunity to determine the confidence of deuterium calculations using a combinatorial strategy. The approach requires DIA-based acquisition methods that are available on most MS platforms, making the switch to HX-MS2 straightforward. Considerable time is saved through auto-validation and more importantly, more complex samples can now be characterized and at higher throughput, as illustrated in a drug binding analysis of the ultralarge kinase DNA-PKcs, isolated directly from mammalian cells.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
SpeciesList scientific name: Oryctolagus cuniculus (Rabbit); NCBI TaxID: 9986; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Eclipse; Q Exactive Plus; TripleTOF 6600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-08-31 13:55:56ID requested
12023-09-08 07:38:03announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: DIA, LC-MS, SWATH-MS, DNA-PK,HX-MS
Contact List
contact affiliationDepartment of Biochemistry & Molecular Biology, University of Calgary, Calgary, AB, Canada
contact emaildschriem@ucalgary.ca
lab head
contact affiliationUniversity of Calgary
contact emailfrantisek.filandr@ucalgary.ca
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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