Sarcoma is a type of cancer that developed from transformed mesenchymal cells like bone, cartilage, fat, muscle, etc. and is broadly categorized into two types, bone and soft tissue sarcoma. Solid tumor cells rely on glycolysis for energy production, contrary to the general belief, it was previously reported that sarcoma cells rely on mitochondrial respiration and the respiratory chain inhibitors drugs such as methylglyoxal, metformin, etc. selectively kill sarcoma cells. Therefore, the study of detailed mitochondrial proteomes is essential to understand the basic metabolic requirement of sarcoma mitochondria. Here, we generate a solid fibrosarcoma mouse tumor model by injecting a chemical carcinogen 3-methyl cholanthrene into the left hind leg. Differential proteomic profiling of ultrapure mitochondria from mice fibrosarcoma tissues (ST, n=4) and its contralateral leg muscle tissues (CLT, n=4) was performed using an LTQ orbitrap XL mass spectrometer. We found ~429 mitochondria-associated protein hits in a total of eight mice tissue samples. Most of the mitochondrial respiratory chain (MRC) subunits were unaltered, whereas two mitochondrial complex IV i.e. cytochrome c oxidase subunits were upregulated.