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PXD044892

PXD044892 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAntimicrobial peptides of the apidaecin family inhibit the bacterial ribosome by multimodal mechanisms involving three different sites on the large ribosomal subunit
DescriptionProline-rich antimicrobial peptides (PrAMPs) inhibit bacterial ribosomes by binding to the polypeptide exit tunnel (PET) near the peptidyl transferase center. Api137, an optimized derivative of honeybee apidaecin, traps the release factor (RF) at the ribosome, thereby arresting the ribosomes at stop codons. This study shows that Api137 occupies a second binding site near the exit of the PET and represses protein translation independently of RF1-trapping. Api88, the C-terminally amidated (-CONH2) analog of Api137 (-COOH), binds in a slightly shifted manner to the same regions without RF trapping, presumably by interfering with the translation process. Api88 also binds to a pocket deep within domain III of the 23S rRNA. In conclusion, Api88 and Api137 inhibit ribosomes through different multimodal mechanisms. These mechanisms have likely reduced the emergence of bacterial resistance and contributed to the evolutionary success of this structurally diverse group of PrAMPs, providing a promising pool for drug development efforts.
HostingRepositoryPanoramaPublic
AnnounceDate2024-06-02
AnnouncementXMLSubmission_2024-06-02_08:15:22.863.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterDaniela Volke
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListDeamidated; Oxidation
InstrumentSYNAPT G2-Si
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-08-27 16:39:58ID requested
12024-06-02 08:15:23announced
Publication List
Lauer SM, Reepmeyer M, Berendes O, Klepacki D, Gasse J, Gabrielli S, Grubm, ü, ller H, Bock LV, Krizsan A, Nikolay R, Spahn CMT, Hoffmann R, Multimodal binding and inhibition of bacterial ribosomes by the antimicrobial peptides Api137 and Api88. Nat Commun, 15(1):3945(2024) [pubmed]
Keyword List
submitter keyword: PrAMPs, 70S ribosome
Contact List
Ralf Hoffmann
contact affiliationUniversität Leipzig
contact emailralf.hoffmann@bbz.uni-leipzig.de
lab head
Daniela Volke
contact affiliationUniversität Leipzig
contact emaildaniela.volke@bbz.uni-leipzig.de
dataset submitter
Full Dataset Link List
Panorama Public dataset URI