The application of treatments to reduce/control the number of Varroa destructor mites within in Apis mellifera colonies is common practice in apiculture. The effectiveness of the treatments in reducing/removing Varroa mites from colonies has been extensively studied, however, the effects of the treatments on the bees within the colonies is lacking. This work utilises proteomics to investigate how the presence of Apivar® strips within hives effects the worker bees. Samples of A. mellifera were isolated from colonies one week before Apivar® treatment and were sampled for 1, 3, 6 and 8 weeks post-treatment application. The results highlight two large proteomic shifts in workers between Control and Week 1 samples in comparison to Weeks 3, 6 and 8 samples. Cuticular proteins were decreased in abundance and translational proteins were increased in abundance in Weeks 3, 6 and 8 in comparison to Control and Week 1 samples. The proteomic shift in Week 8 in comparison to the other treatment samples was not as intense, with a lower number of increased translational proteins, indicating a reduction of treatment effect the longer it is within the hive. The work provides an initial insight into the effects Apivar® has on the honeybee population and how it may contribute to a stressful hive environment to a colony previously infested with Varroa mites.