Five experimental diets were prepared with equal levels of protein (48% crude protein) and lipid (12% crude lipid): a diet based on fishmeal (referred to as Group FM), a diet containing 7% β-conglycinin (referred to as Group 7S), a diet containing 10% glycinin (referred to as Group 11S), a β-conglycinin diet supplemented with 2% alanine-glutamine (referred to as Group CCgGln), and glycinin diet supplemented with 2% alanine-glutamine (referred to as Group GcGln). Healthy hybrid groupers (8.50 ± 0.01 g) were fed each diet for 8 weeks. After the feeding trial finished, distal intestine (DI) samples from the three groups were obtained to determine proteome.