PXD044586

PXD044586 is an original dataset announced via ProteomeXchange.

Title	Sac1 interaction proteomics of ALFA Sac1 and mutants
Description	Sphingolipids are structural membrane components that also function in cellular stress responses. The serine palmitoyl-transferase (SPT) catalyzes the rate limiting step in sphingolipid biogenesis. Its activity is tightly regulated through multiple bind-ing partners, including Tsc3, Orm proteins, ceramides, and the phosphatidylinositol-4-phosphate (PI4P) phosphatase Sac1. The structural organization and regulatory mechanisms of this complex are not yet understood. Here, we report the high-resolution cryo-EM structures of the yeast SPT in complex with Tsc3 and Orm1 (SPOT) as dimers and monomers and a monomeric complex further carrying Sac1 (SPOTS). In all complexes, the tight interaction of the down-stream metabolite ceramide and Orm1 reveals the ceramide dependent inhibition. Additionally, observation of ceramide and ergosterol binding suggests a co-regulation of sphingolipid biogenesis and sterol metabolism within the SPOTS com-plex.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:06:54.069.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Florian Fröhlich
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-15 03:59:20	ID requested
1	2023-10-24 11:00:18	announced
⏵ 2	2023-11-14 09:06:54	announced	2023-11-14: Updated project metadata.

Sch, ä, fer JH, K, ö, rner C, Esch BM, Limar S, Parey K, Walter S, Januliene D, Moeller A, Fr, ö, hlich F, Structure of the ceramide-bound SPOTS complex. Nat Commun, 14(1):6196(2023) [pubmed]

submitter keyword: Sac1

S. cerevisiae

proteomics

Florian Fröhlich
contact affiliation	Osnabrück University Department of Biology/Chemistry Bioanalytical Chemistry section 49076 Osnabrück, Germany
contact email	florian.froehlich@uni-osnabrueck.de
lab head
Florian Fröhlich
contact affiliation	University of Osnabrueck; DEpartment of Biology/Chemistry
contact email	florian.froehlich@uni-osnabrueck.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD044586

PRIDE project URI

• PRIDE
  1. PXD044586
     1. Label: PRIDE project
     2. Name: Sac1 interaction proteomics of ALFA Sac1 and mutants