We knocked out the ChATG8 gene of Colletotrichum higginsianum IMI349063 using Agrobacterium-mediated transformation technology and obtained the Chatg8Δ mutant, and subsequently collected mycelium from the WT strain after 4 days of incubation, the WT strain after 10 days of incubation, and the Chatg8Δ mutant after 10 days of incubation, respectively, for proteome sequencing.