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PXD043968 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBreeding from 1891 to 2010 did not increase the content of amylase/trypsin-inhibitors in wheat (Triticum aestivum L.)
DescriptionThe prevalence of hypersensitivities towards wheat has increased in the last decades. Apart from celiac disease these include allergic and other inflammatory reactions summarized under the term non-celiac wheat sensitivity. One suspected trigger is the family of amylase/trypsin-inhibitors (ATIs), non-gluten proteins that are prominent wheat allergens and that activate the toll-like receptor 4 on intestinal immune cells to promote intestinal and extra-intestinal inflammation. We therefore quantified 13 ATIs in 60 German hexaploid winter wheat cultivars originating from 1891 to 2010 and harvested in three years by targeted liquid chromatography-tandem mass spectrometry combined with stable isotope dilution assay using specific marker peptides as internal standards. The total ATI content and that of the two major ATIs 0.19 and CM3 did not change from old cultivars (first registered from 1891–1950) to modern cultivars (1951–2010). There were also no significant changes in ATI distribution.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportsupported by repository but incomplete data and/or metadata
PrimarySubmitterSabrina Geisslitz
SpeciesList scientific name: Triticum aestivum; NCBI TaxID: 4565;
ModificationListGln->pyro-Glu; Carbamidomethyl; Label:13C(6)15N(2); Label:13C(5)15N(1); Label:13C(6)15N(4); Label:13C(6)15N(1)
InstrumentTSQ Vantage
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-07-20 17:47:57ID requested
12023-08-29 11:46:20announced
22023-09-01 15:13:03announced: Added PubMed Id
Publication List
Geisslitz S, Pronin D, Neerukonda M, Curella V, Neufang S, Koch S, Weichert H, Weber H, B, ö, rner A, Schuppan D, Scherf KA, Breeding from 1891 to 2010 did not increase the content of amylase/trypsin-inhibitors in wheat (Triticum aestivum). NPJ Sci Food, 7(1):43(2023) [pubmed]
Keyword List
submitter keyword: Amylase/trypsin-inhibitors (ATIs), Heritability, non-celiac gluten sensitivity (NCGS), non-celiac wheat sensitivity (NCWS), stable isotope dilution assay (SIDA), wheat allergy
Contact List
Katharina Scherf
contact affiliationKarlsruhe Institute of Technology
contact emailkatharina.scherf@kit.edu
lab head
Sabrina Geisslitz
contact affiliationKarlsruhe Institute of Technology
contact emailsabrina.geisslitz@kit.edu
dataset submitter
Full Dataset Link List
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