In eukaryotes, several gene have been found that ribosomal frameshifting efficiently occurs on its slippery site. However, less study focuses on codon repeat induce frameshifting. To screen whether the codon repeat in HDAC1 gene could induced frameshifting and produce frameshifting peptide. we generate a HDAC1-FS-Flag construct. Detection the HDAC1 frameshifting protein using immunoprecipitation-Mass Spectrometry.