PXD043667-1

PXD043667 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	ProteasomeID: quantitative mapping of proteasome interactomes and substrates for in vitro and in vivo studies
Description	Proteasomes are essential molecular machines responsible for the degradation of proteins in eukaryotic cells. Altered proteasome activity has been linked to neurodegeneration, auto-immune disorders and cancer. Despite the relevance for human disease and drug development, no method currently exists to monitor proteasome composition and interactions in vivo in animal models. To fill this gap, we developed a strategy based on tagging of proteasomes with promiscuous biotin ligases and generated a new mouse model enabling the quantification of proteasome interactions by mass spectrometry. We show that biotin ligases can be incorporated in fully assembled proteasomes without negative impact on their activity. We demonstrate the utility of our method by identifying novel proteasome-interacting proteins, charting interactomes across mouse organs, and showing that proximity-labeling enables the identification of both endogenous and small molecule-induced proteasome substrates.
HostingRepository	MassIVE
AnnounceDate	2024-07-05
AnnouncementXML	Submission_2024-07-05_13:51:22.660.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Leibniz FLI Proteomics
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	Carbamidomethyl; Oxidation
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-11 00:32:10	ID requested
⏵ 1	2024-07-05 13:51:23	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: proteasome, BioID, mass spectrometry, protein degradation, proximity labeling, mouse model

submitter keyword: proteasome, BioID, mass spectrometry, protein degradation, proximity labeling, mouse model

Contact List

Alessandro Ori
contact affiliation	Leibniz Institute on Aging Fritz Lipmann Institute (FLI)
contact email	alessandro.ori@leibniz-fli.de
lab head
Leibniz FLI Proteomics
contact affiliation	Leibniz FLI
contact email	proteomics@leibniz-fli.de
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000092396/