There is a direct interaction relationship between the H. satumaensis and ecdysterone and juvenile hormone. Referring to the hormones concentration level in silkworm, 400 ng/m L concentration of 20E and 250 ng/m L of JH were used to induce the culture of H. satumaensis respectively and then analyzed by transcriptome and proteome. Our results showed that most of the differentially expressed genes induced by hormones interrelated with the PHI, indicating that response to hormones is one of the most important processes in the colonization of fungal insects. The response of the fungi to 20E was mainly focused on the initiation of host infection mechanism and the metabolism of amino sugars and other important biological processes. The differentially expressed proteins in the JH treatment group were mainly involved in the process of ribosome anabolism and protein transcription and translation. In addition, exocrine proteins were also involved in the response, and 45 proteins were co-expressed in the two treatment groups and most of them were up-regulated, which was consistent with the phenotypic phenomenon of increased mucilage thickness.