PXD043351 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The proteome of B cell maturation identifies PDCD4 as limiting the response of marginal zone B cells – WT and PDCD4 KO MZ B cells. Includes Fasta file |
Description | During B cell maturation, transitional and mature B cells acquire cell-intrinsic features that determine their ability to exit quiescence and mount effective immune responses. We used high-resolution mass spectrometry to quantify the proteome of B cell subsets from the mouse spleen and map the differential expression of environmental sensing, transcription- and translation initiation-factors that define cellular identity and function. By comparing transcriptome and proteome, we identified mRNAs linked to B cell activation and differentiation that are expressed without detectable protein. These "poised" mRNAs might enable rapid protein production through increased translation or protein stability. In addition, we found that the translational repressor PDCD4 restrains the response of marginal zone B cells to a T-independent antigen. Our molecular characterization of B cell maturation is a valuable resource to further explore the mechanisms underpinning the specialised functions of B cell subsets. |
HostingRepository | PRIDE |
AnnounceDate | 2023-06-29 |
AnnouncementXML | Submission_2023-06-29_03:41:33.615.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | AndrewHowden |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | acetylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-06-27 15:28:58 | ID requested | |
⏵ 1 | 2023-06-29 03:41:33 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: B cells, PDCD4 |
Contact List
Professor MartinTurner |
contact affiliation | Immunology programme, The Babraham Institute, Cambridge, UK |
contact email | martin.turner@babraham.ac.uk |
lab head | |
AndrewHowden |
contact affiliation | University of Dundee |
contact email | a.howden@dundee.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD043351
- Label: PRIDE project
- Name: The proteome of B cell maturation identifies PDCD4 as limiting the response of marginal zone B cells – WT and PDCD4 KO MZ B cells. Includes Fasta file