PXD043254

PXD043254 is an original dataset announced via ProteomeXchange.

Title	Non - canonical functions of UHRF1 maintain DNA methylation homeostasis in cancer cell
Description	We have generated and validated degron alleles of UHRF1 and/or DNMT1 in several human colorectal cancer cell lines. We then used genomics and bioinformatics to precisely describe he DNA demethylation dynamics in these cells, leading to the conclusion that UHRF1 maintains DNA methylation in cancer cells not only by stimulating DNMT1. Proteomics and genetics lead us to conclude that UHRF1 regulates DNMT3A, DNMT3B and TET2 activity in addition to regulating DNMT1. The tools we have developed will be valuable for future research efforts, and our results advance our understanding of cancer epigenetics, with potentially important therapeutic applications.
HostingRepository	PRIDE
AnnounceDate	2024-02-13
AnnouncementXML	Submission_2024-02-12_21:36:11.910.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Enes Ugur
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-23 15:58:28	ID requested
⏵ 1	2024-02-12 21:36:12	announced

10.1101/2023.07.11.548318;

submitter keyword: UHRF1, DNA methylation, Epigenetics,Chromatin proteome, DNMT1

Pierre-Antoine Defossez
contact affiliation	Université Paris Cité, CNRS, Epigenetics and Cell Fate, F-75013 Paris, France
contact email	pierre-antoine.defossez@cnrs.fr
lab head
Enes Ugur
contact affiliation	Faculty of Biology and Center for Molecular Biosystems (BioSysM), Human Biology and BioImaging, LMU Munich & Department of Proteomics and Signal Transduction, Max-Planck Institute of Biochemistry
contact email	ugur@bio.lmu.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD043254

PRIDE project URI

• PRIDE
  1. PXD043254
     1. Label: PRIDE project
     2. Name: Non - canonical functions of UHRF1 maintain DNA methylation homeostasis in cancer cell