The protein landscapes of LSCC and RSCC were constructed, and the hallmarks of tumorigenesis and characteristics of TME for LSCC and RSCC were identified. These findings improved our understanding of LSCC and RSCC and may facilitate the development of diagnostic or therapeutic targets for LSCC and RSCC. Collected samples were digested as previously described. Briefly, adding appropriate volume of lysate containing 1% protease inhibitor cocktail into the samples. Then, the samples were further ultrasonically lysed (2s-on, 3s-off) for 10 min until the solution became transparent. After centrifugation, supernatant was absorbed into a new EP tube to obtain protein extract. Total protein was quantified by BCA method. After quantification, DTT was added to the sample to make the final concentration of the solution was 5 mM. The EP tube was placed at 56 ℃ for 60 min. After the solution was cooled to room temperature, 55 mM IAM was added to block free sulfhydryl at room temperature for 45 min in the dark. Next, 60 μg