To gain a comprehensive understanding of non-histone methylation during grape berry ripening. A 4D-label free quantitative proteomics approach was used to study the non-histone methylation of lysine during grape berry development and ripening. The ‘Kyoho’ grape berries at EL 31, EL 33, EL 35 and EL 37 were collected from six trees with equal vigor were selected in spring 2022 at the vineyard of the Henan University of Science and Technology, Luoyang, Henan, China (34.66° N, 112.37° E).