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We identified small open reading frames encoded peptided in human serum with nanoparticle protein coronas method using mass spectrometry. Four nanoparticle material treatments (TiO2, Fe3O4@SiO2, Fe3O4@SiO2@TiO2, NPs-mix), ACN and untreated control were performed with triplicates. The comparision of 96 well plate and centrifuge tube was carried out with 6 replicates and 4 replicates respectively.