PXD042246

PXD042246 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	MS for BIRC6 Complex from HEK293F
Description	The plasmids were then transiently transfected into HEK293F expression system for overexpression. After incubation for 48 h, the cells were collected, washed one with ice-cold PBS. The resuspended cells were then lysed by sonication, and the lysate supernatant after centrifugation was incubated with M2-FLAG affinity beads for 1.5 h. After several round of washing with buffer B, the FLAG-affinity proteins were eluted by 0.5 mg/mL FLAG peptide dissolved in buffer C. The elute was further purified by gel filtration (Superose 6 Increase Columns, Cytiva) using buffer C. The fraction at 0.5 mL/tube was collected starting from 7 mL. Concentrate a few tubes from the main peak diagram. The purified FLAG-BIRC6 complex was analyzed by mass spectrometry following separation of the complex by SDS-PAGE. The full lane was sliced into 6 pieces from top to bottom with assigned sample numbers: 1, 2-1, 2-2, 3-1, 3-2, and 4.
HostingRepository	MassIVE
AnnounceDate	2023-05-15
AnnouncementXML	Submission_2023-05-15_17:26:14.809.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	shiyiyi
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Acetyl; Oxidation; Lys->CamCys
Instrument	Q Exactive Plus

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-15 17:16:40	ID requested
⏵ 1	2023-05-15 17:26:15	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: BIRC6, UBA6, Smac

submitter keyword: BIRC6, UBA6, Smac

Contact List

Xiao-Bo Qiu
contact affiliation	Beijing Normal University
contact email	xqiu@bnu.edu.cn
lab head
shiyiyi
contact affiliation	Beijing Normal University
contact email	lss2016410@163.com
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000091953/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000091953/