PXD042110

PXD042110 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Loss of the armadillo-repeat protein Plakophilin 2 in obesity breaks cell cycle dynamics to breed adipocyte senescence
Description	Plakophilin2 (PKP2) is a key component of desmosomes mostly recognized for its involvement in the fibro fatty infiltration of heart muscle under defective PKP2. While thoroughly explored in cardiomyocytes, less attention has been given to its role in fat cells. Here we report steadily increased PKP2 during adipogenesis, with expression levels reaching its apex in terminally differentiated adipocytes. Notably, the loss of this protein in adipocytes under a pro-inflammatory microenvironment demonstrates its commitment in maintaining the expression of genes required to nurture cell cycle. Then, we show that diminished expressions of this member of the armadillo repeat family in subcutaneous adipose tissue co-segregate with obesity, being normalized upon mild to intense weight loss. We also demonstrate that impaired PKP2 in human adipocytes breaks cell cycle dynamics to breed premature senescence, a key rheostat for stress induced adipose tissue dysfunction. Conversely, restoring PKP2 in inflamed adipocytes rewires E2F signalling towards re-activation of cell cycle and decreased senescence. Our findings bound the expression of PKP2 in fat cells to the physiopathology of obesity, and uncover a previously unknown defect in cell cycle and activated adipocyte senescence due to impaired PKP2.
HostingRepository	MassIVE
AnnounceDate	2023-05-10
AnnouncementXML	Submission_2023-05-10_03:51:05.510.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Witold Szymanski
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	CarbamidomethylDTT
Instrument	timsTOF Pro

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-10 00:46:38	ID requested
⏵ 1	2023-05-10 03:51:05	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Plakophilin-2, adipose tissue, adipocytes, senescence, inflammation, obesity

submitter keyword: Plakophilin-2, adipose tissue, adipocytes, senescence, inflammation, obesity

Contact List

Francisco Ortega
contact affiliation	Institut d Investigacio Biomedica de Girona
contact email	fortega@idibgi.org
lab head
Witold Szymanski
contact affiliation	Philipps-University Marburg Biochemical/Pharmacological Center Department of Medicine
contact email	witold.szymanski@uni-marburg.de
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000091898/