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PXD041979

PXD041979 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBlautia producta displays great plasticity of short open reading frame-encoded peptides under different environmental conditions
DescriptionShort open reading frames (sORF) have the potential to encode small proteins in the human gut microbiome, but their role in this environment is rarely understood. These small proteins, known as sORF-encoded peptides (SEP), are less than or equal to 100 amino acids in length. Using bottom-up proteomics (BUP) and top-down proteomics (TDP) approaches, we aimed to identify SEP under various growth conditions and stress exposure in Blautia producta, a key species in mediating colonization resistance and dampening gut inflammation. After applying a rigorous filtering and validation procedure, we identified 45 SEP. Our findings indicate that in contrast to previous results the production of specific SEP is not restricted to direct bacterial interactions within the microbiome but rather depends on growth and stress conditions of the environment. Top-down analysis improved identification confidence and detected a number of full-length with and without N-terminal methionine excision (NME), N- or C-terminal truncated or post-translational modified proteoforms of SEP, indicating that these are common occurrences in B. producta. These findings highlight SEP as a ubiquitous class of non-annotated polypeptides that have been overlooked as a subset of proteins in B. producta.
HostingRepositoryPRIDE
AnnounceDate2023-09-19
AnnouncementXMLSubmission_2023-09-19_01:53:07.865.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAndreasTholey
SpeciesList scientific name: Blautia producta ATCC 27340 = DSM 2950; NCBI TaxID: 1121114;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos; Q Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-05-04 06:01:02ID requested
12023-09-19 01:53:08announced
Publication List
Keyword List
submitter keyword: SEP, top-down proteomics, sORF-encoded peptides, IBD, inflammation, bottom-up proteomics, proteoform, Short open reading frames,Bacterial proteomics
Contact List
AndreasTholey
contact affiliationSystematic Proteome Research & Bioanalytics, Institute for Experimental Medicine, Christian-Albrechts-Universit├Ąt zu Kiel, Kiel, Germany
contact emaila.tholey@iem.uni-kiel.de
lab head
AndreasTholey
contact affiliationSystematic Proteome Research & Bioanalytics, University of Kiel
contact emaila.tholey@iem.uni-kiel.de
dataset submitter
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Dataset FTP location
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