PXD041979

PXD041979 is an original dataset announced via ProteomeXchange.

Title	Blautia producta displays great plasticity of short open reading frame-encoded peptides under different environmental conditions
Description	Short open reading frames (sORF) have the potential to encode small proteins in the human gut microbiome, but their role in this environment is rarely understood. These small proteins, known as sORF-encoded peptides (SEP), are less than or equal to 100 amino acids in length. Using bottom-up proteomics (BUP) and top-down proteomics (TDP) approaches, we aimed to identify SEP under various growth conditions and stress exposure in Blautia producta, a key species in mediating colonization resistance and dampening gut inflammation. After applying a rigorous filtering and validation procedure, we identified 45 SEP. Our findings indicate that in contrast to previous results the production of specific SEP is not restricted to direct bacterial interactions within the microbiome but rather depends on growth and stress conditions of the environment. Top-down analysis improved identification confidence and detected a number of full-length with and without N-terminal methionine excision (NME), N- or C-terminal truncated or post-translational modified proteoforms of SEP, indicating that these are common occurrences in B. producta. These findings highlight SEP as a ubiquitous class of non-annotated polypeptides that have been overlooked as a subset of proteins in B. producta.
HostingRepository	PRIDE
AnnounceDate	2023-09-19
AnnouncementXML	Submission_2023-09-19_01:53:07.865.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	AndreasTholey
SpeciesList	scientific name: Blautia producta ATCC 27340 = DSM 2950; NCBI TaxID: 1121114;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-04 06:01:02	ID requested
⏵ 1	2023-09-19 01:53:08	announced

submitter keyword: SEP, top-down proteomics, sORF-encoded peptides, IBD, inflammation, bottom-up proteomics, proteoform, Short open reading frames,Bacterial proteomics

AndreasTholey
contact affiliation	Systematic Proteome Research & Bioanalytics, Institute for Experimental Medicine, Christian-Albrechts-Universität zu Kiel, Kiel, Germany
contact email	a.tholey@iem.uni-kiel.de
lab head
AndreasTholey
contact affiliation	Systematic Proteome Research & Bioanalytics, University of Kiel
contact email	a.tholey@iem.uni-kiel.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041979
     1. Label: PRIDE project
     2. Name: Blautia producta displays great plasticity of short open reading frame-encoded peptides under different environmental conditions