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PXD041966
PXD041966 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Pick-up single-cell proteomic analysis for quantifying up to 3000 proteins in a tumor cell |
| Description | The shotgun proteomic analysis is currently the most promising single-cell protein sequencing technology, however its identification level of ~1000 proteins per cell is still insufficient for practical applications. Here, we develop a pick-up single-cell proteomic analysis (PiSPA) workflow to achieve a deep identification capable of quantifying up to 3000 protein groups in a tumor cell using the label-free quantitative method. The PiSPA workflow is specially established for single-cell samples mainly based on a nanoliter-scale microfluidic liquid handling robot, capable of achieving single-cell capture, pretreatment and injection under the pick-up operation strategy. Using this customized workflow with remarkable improvement in protein identification, 1804–3349, 1778–3049 and 1074–2487 protein groups are quantified in single A549 cells (n = 37), HeLa cells (n = 44) and U2OS cells (n = 27), respectively. Benefiting from the flexible cell picking-up ability, we study tumor cell migration at the single cell proteome level, demonstrating the potential in practical biological research from single-cell insight. |
| HostingRepository | iProX |
| AnnounceDate | 2023-10-30 |
| AnnouncementXML | Submission_2024-02-12_04:36:15.867.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yu Wang |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-05-03 20:59:29 | ID requested | |
| ⏵ 1 | 2024-02-12 04:36:16 | announced |
Publication List
Keyword List
| submitter keyword: single-cell proteomics, microfluidics, cell migration, shotgun proteomics |
Contact List
| Qun Fang | |
|---|---|
| contact affiliation | Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University |
| contact email | fangqun@zju.edu.cn |
| lab head | |
| Yu Wang | |
| contact affiliation | Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University |
| contact email | wangyu310@zju.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
