PXD041795 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | LC-MS/MS of HEK-293 inducibly expressing TDP-43 protein (WT and 3 non-functional mutants) |
Description | Aggregation of the RNA-binding protein TDP-43 is the key neuropathological feature of neurodegenerative diseases, including ALS and FTLD. TDP-43 is a ubiquitously expressed, nucleic acid-binding protein essential for human development. In physiological conditions, TDP-43 is predominantly nuclear, forms physiological oligomers and performs a wide variety of functions in the metabolism of RNA. However, whether oligomerization is required for TDP-43 functionality and whether oligomerization and RNA binding are intertwined, and if so how, remains poorly understood. To study this question, we generated three GFP-tagged human TDP-43 variants with either six point mutations that disrupt TDP-43 oligomerization (6M), five point mutations within the RNA-recognition motifs that disrupt RNA binding (RBDm) or both (6M&RBDm), and developed the corresponding four (TDP-43 WT and 3 mutants) isogenic HEK-293 cell lines expressing a single copy of GFP-TDP-43 under the control of a doxycycline-inducible promoter. Here, we induced the expression of all four TDP-43 constructs and studied the effects of the mild overexpression of the different TDP-43 variants on the human cell proteome. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:02:19.518.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Manuela Pérez Berlanga |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-04-25 04:06:20 | ID requested | |
1 | 2023-08-31 07:03:26 | announced | |
2 | 2023-11-14 09:04:58 | announced | 2023-11-14: Updated project metadata. |
⏵ 3 | 2024-10-22 06:02:20 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.15252/embj.2022111719; |
P, é, rez-Berlanga M, Wiersma VI, Zbinden A, De Vos L, Wagner U, Foglieni C, Mallona I, Betz KM, Cl, é, ry A, Weber J, Guo Z, Rigort R, de Rossi P, Manglunia R, Tantardini E, Sahadevan S, Stach O, Hruska-Plochan M, Allain FH, Paganetti P, Polymenidou M, Loss of TDP-43 oligomerization or RNA binding elicits distinct aggregation patterns. EMBO J, 42(17):e111719(2023) [pubmed] |
Keyword List
submitter keyword: Human, kidney, TDP-43, LC-MS/MS |
Contact List
Magdalini Polymenidou |
contact affiliation | Polymenidou group Department of Quantitative Biomedicine University of Zurich Switzerland |
contact email | magdalini.polymenidou@uzh.ch |
lab head | |
Manuela Pérez Berlanga |
contact affiliation | University of Zurich |
contact email | manuela.perezberlanga@uzh.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD041795
- Label: PRIDE project
- Name: LC-MS/MS of HEK-293 inducibly expressing TDP-43 protein (WT and 3 non-functional mutants)