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PXD041795

PXD041795 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLC-MS/MS of HEK-293 inducibly expressing TDP-43 protein (WT and 3 non-functional mutants)
DescriptionAggregation of the RNA-binding protein TDP-43 is the key neuropathological feature of neurodegenerative diseases, including ALS and FTLD. TDP-43 is a ubiquitously expressed, nucleic acid-binding protein essential for human development. In physiological conditions, TDP-43 is predominantly nuclear, forms physiological oligomers and performs a wide variety of functions in the metabolism of RNA. However, whether oligomerization is required for TDP-43 functionality and whether oligomerization and RNA binding are intertwined, and if so how, remains poorly understood. To study this question, we generated three GFP-tagged human TDP-43 variants with either six point mutations that disrupt TDP-43 oligomerization (6M), five point mutations within the RNA-recognition motifs that disrupt RNA binding (RBDm) or both (6M&RBDm), and developed the corresponding four (TDP-43 WT and 3 mutants) isogenic HEK-293 cell lines expressing a single copy of GFP-TDP-43 under the control of a doxycycline-inducible promoter. Here, we induced the expression of all four TDP-43 constructs and studied the effects of the mild overexpression of the different TDP-43 variants on the human cell proteome.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:02:19.518.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterManuela Pérez Berlanga
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-04-25 04:06:20ID requested
12023-08-31 07:03:26announced
22023-11-14 09:04:58announced2023-11-14: Updated project metadata.
32024-10-22 06:02:20announced2024-10-22: Updated project metadata.
Publication List
10.15252/embj.2022111719;
P, é, rez-Berlanga M, Wiersma VI, Zbinden A, De Vos L, Wagner U, Foglieni C, Mallona I, Betz KM, Cl, é, ry A, Weber J, Guo Z, Rigort R, de Rossi P, Manglunia R, Tantardini E, Sahadevan S, Stach O, Hruska-Plochan M, Allain FH, Paganetti P, Polymenidou M, Loss of TDP-43 oligomerization or RNA binding elicits distinct aggregation patterns. EMBO J, 42(17):e111719(2023) [pubmed]
Keyword List
submitter keyword: Human, kidney, TDP-43, LC-MS/MS
Contact List
Magdalini Polymenidou
contact affiliationPolymenidou group Department of Quantitative Biomedicine University of Zurich Switzerland
contact emailmagdalini.polymenidou@uzh.ch
lab head
Manuela Pérez Berlanga
contact affiliationUniversity of Zurich
contact emailmanuela.perezberlanga@uzh.ch
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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