This project is “Medical proteome analysis of osteoporosis- and bone mass-related proteins using the Kibo Japanese Experiment Module of International Space Station (ISS)”. Utilizing proteomic technologies, this research aims to identify proteins involved in space flight-induced osteopenia as well as osteoporosis occurring on the ground by searching serum, and skeletal muscle and bone (femoral diaphysis, mandible) proteomes. The goal of this study is to get better understanding to protect the health of both astronauts and osteoporosis patients. Proteome analyses of C57BL/6J space flown mouse (micro-G and artificial 1-G [n = 5/group, in femoral diaphysis and n = 6/group in mandible]) were carried out tryptic digestion followed by LC-MS/MS analysis (DIA). The obtained results were analyzed using Spectronaut Pulsar X against the mouse-bone spectral library for identification and quantification of proteins.