Seven health subjects took fish oil supplements (1.125 mg DHA and 875 mg EPA) for 30 days.Prior to supplementation, at 30 day of supplementation and after a 30 day washout period patient plasma was collected. From subject plasma HDL was isolated using a two step ultracentrifugation procced. The isolated HDL was then used in cholesterol efflux assay, anti-inflammatory capacity assay, proteomics and lipidomics.