Background: Toxocara canis is a prevalent helminth, which threatens millions of people worldwide with a predilection for pediatric and adolescent populations in impoverished communities. Exploring the invasion and developmental mechanisms associated with T. canis infection in its definitive hosts will help to better control and eliminate the effects of toxocariasis. Methods: To investigate the molecular mechanism of Beagle dogs' response to T. canis infection, proteomic alterations in the lung of Beagle puppies were systematically analyzed by data-independent acquisition (DIA)-based quantitative proteomic technology at 96 h post-infection (hpi). Proteins with P-values < 0.05 and fold change> 1.5 or  <0.67 were considered as proteins with differential abundance (PDAs). Results: A total of 28 down-regulated PDAs and 407 up-regulated PDAs were identified at 96 hpi, including RhoC, TM4SFs and LPCAT1, which could be associated with the maintenance and repair of lung homeostasis. Gene Ontology (GO) annotation and K