Updated project metadata. Current protocols used to extract and purify histones are notoriously tedious, especially when using yeast cells. Here, we describe the use of a simple filter-aided sample preparation approach enabling histone extraction from yeast and mammalian cells using acidified ethanol, a condition that not only improves extraction but also inactivates histone-modifying enzymes. We show that our improved method preserves the integrity of histones, and prevents N-terminal clipping of H3, an artefact frequently observed in yeast cells using standard histone extraction protocols. Our method is scalable and provides efficient recovery of histones when extracts are prepared from as little as two million yeast cells. We further demonstrate the application of this approach for the analysis of histone modifications including fungal clinical isolates available in limited quantity. Altogether, this method enables the study of histones and their modifications in a faster, simpler, and more robust manner.