PXD041264

PXD041264 is an original dataset announced via ProteomeXchange.

Title	NAIA for cysteine profiling in HepG2 cells, protein target identification of covalent ligand and studying cysteine oxidative modifications
Description	New cysteine probes, N-acryloylindole-alkynes (NAIAs), have been developed. We have applied NAIA-5 for cysteine profiling in HepG2 cell lysates, and the results have been compared to the conventional cysteine probe iodoacetamide-alkyne (IAA). NAIA-5 allows identification of more ligandable Cys than IAA in our experiment, and more interesting, a unique pool of Cys which have not been profiled previously by any reported cysteine probe. We have further utilized NAIA-5 for covalent ligand screening experiment. This allows us to discover hit compounds targeting the newly identified cysteines and proteins. One of the hit compounds, CL1, demonstrated good bioactivity by arresting cell cycle at G1 phase through targeting proteins associated with cell cycle and regulations. In addition, NAIA-5 has been successfully coupled with NAI compound for studying cysteine oxidative modifications in HepG2 cells by MS experiments.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:48:05.712.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Clive Yik-Sham CHUNG
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-01 01:27:46	ID requested
1	2023-06-14 02:47:53	announced
2	2023-11-14 09:00:58	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:48:06	announced	2024-10-22: Updated project metadata.

10.1038/S41467-023-39268-W;

submitter keyword: Profiling ligandable cysteines and studying cysteine oxidative modifications in human liver cancer cells HepG2 by NAIA-5 using Bruker timsTOF MS

Clive Yik-Sham CHUNG
contact affiliation	Assistant Profssor, School of Biomedical Sciences and Department of Pathology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam Road, Hong Kong, P. R. China
contact email	cyschung@hku.hk
lab head
Clive Yik-Sham CHUNG
contact affiliation	The University of Hong Kong
contact email	cyschung@hku.hk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD041264

PRIDE project URI

• PRIDE
  1. PXD041264
     1. Label: PRIDE project
     2. Name: NAIA for cysteine profiling in HepG2 cells, protein target identification of covalent ligand and studying cysteine oxidative modifications