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PXD041024 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleVEGFR2 pY1173 binding partners as identified by shotgun proteomics
DescriptionThe main goal of the project was to identify proteins binding to vascular endothelial growth factor receptor 2 (VEGFR2) phosphorylation site Y1173. Synthetic peptides, phosphorylated or not, covering different tyrosine phosphorylation sites in VEGFR2 were immobilized and incubated with cell lysates from human umbilical vein endothelial cells. Retained proteins were analyzed by mass spectrometry. Proteins specifically binding to pY1173 peptide were categorized with regard to the presence of an Src Homology 2 (SH2) domain and the main hits were validated in intact cells treated or not with VEGF, for their ability to bind to the activated wild type VEGFR2 but not to a mutant Y1173F VEGFR2. The role of the pY1173 binding partners in VEGF-regulated endothelial biology was further examined in vitro and in vivo.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-03-22 03:21:49ID requested
12023-09-19 05:25:51announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: SH2 domain-binding, LC-MS,VEGF, VEGFR2 pY1173
Contact List
contact affiliationDept. Oncology Pathology, Karolinska Institutet, and Scilifelab, Stockholm, Sweden
contact emailjanne.lehtio@ki.se
lab head
contact affiliationKarolinska Institutet
contact emailgeorgios.mermelekas@scilifelab.se
dataset submitter
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Dataset FTP location
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