PXD040926

PXD040926 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Spatiotemporal profiling of membrane proteins involved in organelle communication
Description	Organelle communication is largely mediated by proteins at membrane contact sites (MCS), such as the endoplasmic reticulum (ER)-mitochondria, ER-plasma membrane (PM), and mitochondria-lipid droplets (LD). In this project, we developed a new tool for identifying membrane proteins at MCS by utilizing two mutually orthogonal binding pair systems, streptavidin-biotin (SA-Bt) and cucurbit[7]uril-adamantane (CB[7]-Ad), in conjunction with two distinct engineered enzymes, TurboID and APEX2. This enabled us to successfully identify proteins at the contact sites of the ER and mitochondria, and further allowed us to detect protein sets that undergo structural and locational changes at the ER-mitochondria contact site during the process of mitophagy.
HostingRepository	jPOST
AnnounceDate	2024-01-27
AnnouncementXML	Submission_2024-01-27_00:56:04.040.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD040926
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ara Lee
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: cellular organisms; NCBI TaxID: 131567;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; alpha-amino acetylated residue; unknown modification; unknown modification
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-17 00:03:11	ID requested
⏵ 1	2024-01-27 00:56:04	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Proteomics, Proximity labeling, Host-guest chemistry, noncovalent binding pairs, cucurbiturils

submitter keyword: Proteomics, Proximity labeling, Host-guest chemistry, noncovalent binding pairs, cucurbiturils

Contact List

Kimoon Kim
lab head
Ara Lee
contact affiliation	Pohang University of Science and Technology
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002091/