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PXD040882 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCell cycle-dependent gene networks for cell proliferation activated by nuclear CK2 complexes
DescriptionProtein kinase CK2 is a conserved serine/threonine kinase that participates in various cellular processes. Elevated nuclear expression of CK2α has been observed in human carcinomas, but mechanisms for its variable localization in cells are poorly understood. This study demonstrates a functional connection between nuclear CK2 and gene expression in relation to cell proliferation. Growth stimulation of quiescent human fibroblasts identified a pool of CK2 highly phosphorylated at serine 7 of the α subunit that translocated into the nucleus. This phosphorylation appeared essential for its nuclear localization and catalytic activity. Protein signatures associated with nuclear CK2 complexes revealed enrichment of transcription factors and chromatin remodelers that seemed unique during progression through G1 phase of the cell cycle. Chromatin immunoprecipitation-sequencing profiling demonstrated recruitment of CK2α to the active gene locus, more abundantly in late G1 phase than in early G1. Notably, we confirmed the presence of CK2α at transcriptional start sites of core histone genes, growth stimulus-associated genes, and ribosomal RNAs. This study suggests that nuclear CK2α complexes, uniquely constituted during cell cycle progression, are essential for cell proliferation, by activating histone genes, triggering ribosome biogenesis, and then synthesize the components necessary for cell division, specified in the nucleus and also in the nucleolus. 
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTakeshi Shibata
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; O-phospho-L-serine; O-phospho-L-threonine
InstrumentTripleTOF 5600; LTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-03-14 23:14:05ID requested
12023-10-21 10:34:51announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: CK2, cell cycle, ChIP-Seq, ChIP-qPCR, gene activation, growth-associated genes, histone genes, nano-LC mass spectrometry, nuclear localization, transcription
Contact List
Miwako Kato Homma
lab head
Takeshi Shibata
contact affiliationSciex
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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