Updated project metadata. Apicomplexan parasites are ubiquitous pathogens of humans. Toxoplasma gondii, a model apicomplexan, possesses a divergent ortholog of mammalian PDK1. This ortholog, TgSPARK, is essential for the ability of parasites to invade and exit their host. Here, we conduct a quantitative mass spectrometry experiment to measure changes to the parasite phosphoproteome following 0, 3, 8, and 24 hours of conditional SPARK depletion.