PXD040536

PXD040536 is an original dataset announced via ProteomeXchange.

Title	A strategy to characterize the global histone PTM landscape within tissues of non-model organisms
Description	Histone post-translational modifications (PTMs) are epigenetic marks that play a critical role in the expression and maintenance of DNA, but they remain largely uninvestigated in non-model organisms due to technical challenges. To begin alleviating this issue, we developed a workflow for histone PTM analysis in the Mozambique tilapia (Oreochromis mossambicus), being a widespread and environmentally hardy fish, using mass spectrometry methods. By incorporating multiple protein digestion methods into the preparation of each sample, we reliably quantified 503 biologically relevant histone PTMs. All of these histone PTMs, collectively referred to as the global histone PTM landscape, were characterized in the gills, kidney, and testes of this fish. By comparing the global histone PTM landscape between the three tissues, we found that 90.46% of histone PTMs were tissue-dependent. The workflow and tools for histone PTM analysis described in this study are now publicly available and enable comprehensive investigation into the influence of environmental stress on histone PTMs in non-model organisms. Given the functionality and flexibility of histone PTMs, we anticipate that the study of histone PTMs in ecologically relevant contexts will provide ground-breaking insights into comparative physiology and evolution.
HostingRepository	PanoramaPublic
AnnounceDate	2024-02-29
AnnouncementXML	Submission_2024-02-29_21:03:54.400.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Elizabeth Mojica
SpeciesList	scientific name: Oreochromis mossambicus; NCBI TaxID: 8127;
ModificationList	Dehydrated; Cation:Na; Carboxyethyl; Carbamidomethyl; Oxidation; Phospho; ADP-Ribosyl; Acetyl; Deamidated; Gln->pyro-Glu; Dioxidation; Delta:H(4)C(2); Carbamyl; GG; Gly; Malonyl; Propionamide; Dimethyl; Methyl; Propionyl; HNE; Trimethyl; Delta:H(4)C(2)O(-1)S(1); Cation:Cu[I]; Cation:Fe[II]; Cation:Ca[II]; Delta:H(8)C(6)O(2); Xlink:EGS[115]; Carboxymethyl; Biotin; Formyl; Amidated; Butyryl; Delta:H(4)C(3)O(1)
Instrument	impact II

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-01 15:39:44	ID requested
1	2023-03-05 13:51:58	announced
⏵ 2	2024-02-29 21:03:55	announced	: Added PubMed Id

Mojica EA, K, ü, ltz D, A Strategy to Characterize the Global Landscape of Histone Post-Translational Modifications Within Tissues of Nonmodel Organisms. J Proteome Res, 23(8):2780-2794(2024) [pubmed]

submitter keyword: Mass spectrometry, epigenetics, histone post-translational modifications, Mozambique tilapia, tissue specificity

Dietmar Kültz
contact affiliation	University of California, Davis
contact email	dkueltz@ucdavis.edu
lab head
Elizabeth Mojica
contact affiliation	University of California, Davis
contact email	eajarett@ucdavis.edu
dataset submitter

Panorama Public dataset URI