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PXD040536 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA strategy to characterize the global histone PTM landscape within tissues of non-model organisms
DescriptionHistone post-translational modifications (PTMs) are epigenetic marks that play a critical role in the expression and maintenance of DNA, but they remain largely uninvestigated in non-model organisms due to technical challenges. To begin alleviating this issue, we developed a workflow for histone PTM analysis in the Mozambique tilapia (Oreochromis mossambicus), being a widespread and environmentally hardy fish, using mass spectrometry methods. By incorporating multiple protein digestion methods into the preparation of each sample, we reliably quantified 503 biologically relevant histone PTMs. All of these histone PTMs, collectively referred to as the global histone PTM landscape, were characterized in the gills, kidney, and testes of this fish. By comparing the global histone PTM landscape between the three tissues, we found that 90.46% of histone PTMs were tissue-dependent. The workflow and tools for histone PTM analysis described in this study are now publicly available and enable comprehensive investigation into the influence of environmental stress on histone PTMs in non-model organisms. Given the functionality and flexibility of histone PTMs, we anticipate that the study of histone PTMs in ecologically relevant contexts will provide ground-breaking insights into comparative physiology and evolution.
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterElizabeth Mojica
SpeciesList scientific name: Oreochromis mossambicus; NCBI TaxID: 8127;
ModificationListDehydrated; Cation:Na; Carboxyethyl; Carbamidomethyl; Oxidation; Phospho; ADP-Ribosyl; Acetyl; Deamidated; Gln->pyro-Glu; Dioxidation; Delta:H(4)C(2); Carbamyl; GG; Gly; Malonyl; Propionamide; Dimethyl; Methyl; Propionyl; HNE; Trimethyl; Delta:H(4)C(2)O(-1)S(1); Cation:Cu[I]; Cation:Fe[II]; Cation:Ca[II]; Delta:H(8)C(6)O(2); Xlink:EGS[115]; Carboxymethyl; Biotin; Formyl; Amidated; Butyryl; Delta:H(4)C(3)O(1)
Instrumentimpact II
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-03-01 15:39:44ID requested
12023-03-05 13:51:58announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Mass spectrometry, epigenetics, histone post-translational modifications, Mozambique tilapia, tissue specificity
Contact List
Dietmar K├╝ltz
contact affiliationUniversity of California, Davis
contact emaildkueltz@ucdavis.edu
lab head
Elizabeth Mojica
contact affiliationUniversity of California, Davis
contact emaileajarett@ucdavis.edu
dataset submitter
Full Dataset Link List
Panorama Public dataset URI