Update information. The project investigated the role of CHTOP in the regulation of neuronal cell death after brain injury. To investigate the mechanism of CHTOP in the regulation of neuronal death, we performed immunoprecipitation to pulldown CHTOP interacting proteins in both neurons and other brain resident cells. The we performed silver staining to show the difference bands between IgG and CHTOP-pulled proteins. The target bands were them cut and send to MS analysis. The peptides were then analyzed by the Thermo ScientificTM Q Exactive mass spectrometer.