We carried out immunoprecipitation-mass spectrometry (IP-MS) analysis to identify the protein components of PGL granules in heat-stressed embryos. GFP::PGL-3 proteins were immunoprecipitated from extracts of embryos grown at 26 oC and the interacting proteins were identified by LC-MS/MS. Proteins involved in translation and RNA control factors (e.g. RNA binding proteins, ribonucleases, RNA helicases, proteins involved in siRNA-mediated mRNA turnover) were enriched in the GFP::PGL-3 co-immunoprecipitants