PXD040098

PXD040098 is an original dataset announced via ProteomeXchange.

Title	Interactors of trapped protein from mitochondria from HeLa cells with stalled import TMT-MS
Description	This study explores the consequences of import failure in mammalian cells; wherein, blocking the import machinery has profound effects on mitochondrial ultra-structure and dynamics, but, surprisingly, does not impact import. The explanation is an astonishing response involving intercellular mitochondrial transfer via tunnelling nanotubes: for the import of healthy mitochondria and jettisoning of those with jammed import sites. These observations support the existence of a widespread mechanism for the rescue of mitochondrial protein import failure. Through proteomics, we identified proteins that interact with the trapped precursor, and may be involved in TNT signalling and formation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:42:25.387.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hope Needs
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-13 10:28:09	ID requested
1	2024-05-24 01:04:58	announced
⏵ 2	2024-10-22 06:42:26	announced	2024-10-22: Updated project metadata.

10.1038/s41467-024-45283-2;

Needs HI, Glover E, Pereira GC, Witt A, H, ü, bner W, Dodding MP, Henley JM, Collinson I, Rescue of mitochondrial import failure by intercellular organellar transfer. Nat Commun, 15(1):988(2024) [pubmed]

submitter keyword: Mitochondria, HeLa, Import, Galactose

Ian Collinson
contact affiliation	School of Biochemistry, University of Bristol
contact email	ian.collinson@bristol.ac.uk
lab head
Hope Needs
contact affiliation	University of Bristol
contact email	hn17563@bristol.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD040098

PRIDE project URI

• PRIDE
  1. PXD040098
     1. Label: PRIDE project
     2. Name: Interactors of trapped protein from mitochondria from HeLa cells with stalled import TMT-MS