PXD039868

PXD039868 is an original dataset announced via ProteomeXchange.

Title	Comprehensive genome-scale and pathway engineering for production of the sustainable aviation fuel in Pseudomonas putida
Description	Biological production of aviation fuels has a realistic impact on global warming. Important sustainable aviation fuel (SAF) targets are emerging and isoprenol is recently identified as a precursor for a promising SAF compound DMCO (1,4-dimethylcyclooctane). Isoprenol has been produced in several engineered microorganisms, and recently, Pseudomonas putida has gained interest as a promising host for isoprenol bioproduction as it can utilize carbon sources generated from inexpensive plant biomass. Here, we engineer metabolically versatile host P. putida for isoprenol production. We employ two computational modeling approaches (Bilevel optimization and Constrained Minimal Cut Sets) to predict gene knockout targets and optimize the “IPP-bypass” pathway in P. putida to maximize isoprenol production. Altogether, the highest isoprenol production titer from P. putida was achieved at 3.5 g/L under fed-batch conditions. This combination of computational and biological engineering on P. putida for an advanced biofuels production has vital significance in enabling a bioproduction process that can use renewable carbon streams.
HostingRepository	PanoramaPublic
AnnounceDate	2025-08-19
AnnouncementXML	Submission_2025-08-19_18:22:54.740.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Chris Petzold
SpeciesList	scientific name: Pseudomonas putida KT2440; NCBI TaxID: 160488;
ModificationList	Carbamidomethyl
Instrument	6460 Triple Quadrupole LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-05 13:57:05	ID requested
⏵ 1	2025-08-19 18:22:55	announced

Banerjee D, Yunus IS, Wang X, Kim J, Srinivasan A, Menchavez R, Chen Y, Gin JW, Petzold CJ, Martin HG, Magnuson JK, Adams PD, Simmons BA, Mukhopadhyay A, Kim J, Lee TS, Genome-scale and pathway engineering for the sustainable aviation fuel precursor isoprenol production in Pseudomonas putida. Metab Eng, 82():157-170(2024) [pubmed]

submitter keyword: Pseudomonas putida, SAF, metabolic engineering, synthetic biology

Chris Petzold
contact affiliation	Lawrence Berkeley National Laboratory
contact email	jbei.panorama@gmail.com
lab head
Chris Petzold
contact affiliation	Lawrence Berkeley National Laboratory
contact email	cjpetzold@lbl.gov
dataset submitter

Panorama Public dataset URI