PXD039865

PXD039865 is an original dataset announced via ProteomeXchange.

Title	EPLIN-Beta is a novel substrate of ornithine decarboxylase antizyme 1, mediating cellular migration
Description	Polyamines promote cellular proliferation, and their levels are controlled by ornithine decarboxylase antizyme 1 (Az1), through the proteasome-mediated, ubiquitin-independent degradation of ornithine decarboxylase (ODC), the rate-limiting enzyme of polyamine biosynthesis. Az1-mediated degradation of other substrates such as cyclin D1, DNp73 and Mps1 also regulate cell growth and centrosome amplification. Collectively, the currently known six Az1-substrates are all linked with tumorigenesis. To understand if Az1-mediated protein degradation might play a key role in regulating cellular process associated with tumorigenesis, we employed quantitative proteomics to identify novel Az1 substrates. Here, we describe the identification of LIM domain and Actin-binding protein 1 (LIMA1), also known as epithelial protein lost in neoplasm (EPLIN), as a new target of Az1. Interestingly, between the two isoforms of EPLIN-Alpha and -Beta, only EPLIN-Beta is the substrate of Az1, degraded in an ubiquitination-independent manner. Absence of Az1 led to elevated EPLIN-Beta levels, which caused enhanced cellular migration of Az1-/- cells. Consistently, higher levels of LIMA1 correlated with poorer overall survival of colorectal cancer patients. Taken together, this study identifies EPLIN-Beta as a novel Az1 substrate regulating cellular migration.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:47:17.049.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Suat Peng Neo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-04 13:57:34	ID requested
1	2023-06-07 20:49:16	announced
⏵ 2	2024-10-22 05:47:17	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: ubiquitin-independent degradation,Antizyme, cell migration, EPLIN/LIMA1

Jayantha Gunaratne
contact affiliation	TBP Lab, Institute of Molecular & Cellular Biology, Agency for Science, Technology and Research (A*STAR), Singapore
contact email	jayanthag@imcb.a-star.edu.sg
lab head
Suat Peng Neo
contact affiliation	Institute of Molecular and Cell Biology (ASTAR)
contact email	neosp@imcb.a-star.edu.sg
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD039865
     1. Label: PRIDE project
     2. Name: EPLIN-Beta is a novel substrate of ornithine decarboxylase antizyme 1, mediating cellular migration