PXD039854

PXD039854 is an original dataset announced via ProteomeXchange.

Title	Identification of TTC29 protein partners in mouse testis
Description	We previously identified gene variants in TTC29 that induce human male infertility due to asthenozoospermia and MMAF phenotype (Lores et al. Am J hum genet 2019). By means of CRISPR/Cas9 mutagenesis we demonstrated that the orthologous TTC29 protein in mouse also constitute a flagellar protein that is required for proper beating of the flagella, indicating an evolutionary-conserved function of TTC29. In here, we took advantage of the mutant mouse model to further decipher the protein complexes associated with TTC29 function within the flagella. We performed co-immunoprecipitation experiments on testis protein extracts from wild-type mice using TTC29 specific antibodies and performed comparative mass spectrometry analyses with two TTC29 KO mouse lines we previously generated and shown to lack TTC29 protein (Ttc29-/- L5 and L7) (Lores et al. Am J hum genet 2019. Mass spectrometry analyses were performed on four independent experimental replicates for each mouse line.
HostingRepository	PRIDE
AnnounceDate	2023-11-06
AnnouncementXML	Submission_2023-11-06_07:28:47.217.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JohannaBRUCE
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-03 15:37:22	ID requested
⏵ 1	2023-11-06 07:28:47	announced

Dataset with its publication pending

submitter keyword: co-immunoprecipitation, asthenozoospermia, male infertility,TTC29, Crispr mouse model

PatrickLORES
contact affiliation	Université de Paris, Institut Cochin, U1016, INSERM, CNRS, Paris, France.
contact email	patrick.lores@inserm.fr
lab head
JohannaBRUCE
contact affiliation	Proteom’IC facility, Université Paris Cité, CNRS, INSERM, Institut Cochin, F-75014 PARIS
contact email	johanna.bruce@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD039854

PRIDE project URI

• PRIDE
  1. PXD039854
     1. Label: PRIDE project
     2. Name: Identification of TTC29 protein partners in mouse testis