In this study, we examined the kidney proteome of Labeo rohita infected intraperitoneally with Edwardsiella tarda (Et). A comparative proteomic analysis of Control and Et infected kidney fwas performed. High-resolution mass spectrometry was used for gut data using label-free quantification. Discovery based proteomics data could identify a panel of differentially expressed proteins (DEPs) between Control and ET condition (Et infected group). Protein abundance changes in a few selected DEPs was then validated using selected/multiple reaction monitoring (S/MRM) approach.