PXD039550

PXD039550 is an original dataset announced via ProteomeXchange.

Title	Identification of Zuo1 interactors upon rapamycin stress
Description	Proteostasis is tightly regulated by TORC1 to meet cellular requirements. Upon TORC1 inhibition, degradative activity is increased, and protein synthesis is reduced through inhibition of translation initiation, to maintain cell viability. Here, we show that the ribosome-associated complex (RAC)/Ssb chaperone system is required to maintain proteostasis and cell viability under TORC1 inhibition, in yeast. In the absence of the Hsp40 cochaperone Zuo1, translation does not decrease in response to loss of TORC1 activity. The functional interaction between Zuo1 and its Hsp70 partner, Ssb is required for proper translational control and proteostasis maintenance upon TORC1 inhibition. Further, we have found that the rapid degradation of eIF4G following TORC1 inhibition is prevented in zuo1Δ cells, contributing to decreased survival in these conditions. Our findings suggest a new role for RAC/Ssb in regulating translation in response to changes in TORC1 signalling.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:29:54.203.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frederic Lamoliatte
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-18 16:26:56	ID requested
1	2023-09-11 04:07:51	announced
⏵ 2	2023-11-14 08:29:54	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: LC-MSMS,Yeast, TMT-labelling, Immunoprecipitation, Co-chaperones

Adrien Rousseau
contact affiliation	Medical Research Council Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, Dundee, Scotland
contact email	a.rousseau@dundee.ac.uk
lab head
Frederic Lamoliatte
contact affiliation	University of Dundee
contact email	flamoliatte001@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD039550
     1. Label: PRIDE project
     2. Name: Identification of Zuo1 interactors upon rapamycin stress