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PXD039470-1

PXD039470 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEnrichment and mass spectrometry analysis of TbTAX-1-interacting or TbTTC29-interacting proteins by co-immunoprecipitation
DescriptionStudy of Trypanosoma ZMYND12’s ortholog (TbTAX-1) confirmed an axonemal localization of the protein and knockdown of TbTAX-1 using RNAi induced dramatic flagellar motility defects as observed in human. Co-immunoprecipitation assays and ultra-structure expansion microscopy in Trypanosoma brucei then formally demonstrated that ZMYND12 and TTC29 directly interact and belong to the same axonemal complex. Subsequent comparative proteomics analysis in Trypanosoma and Ttc29 KO mice models also identified DNAH1 as a new interacting protein of both ZMYND12 and TTC29 proteins. These data evidenced the critical role of ZMYND12 for flagellum function and assembly in humans and Trypanosoma through its interaction with other axonemal partners such as TTC29 and DNAH1 in the same complex.
HostingRepositoryPRIDE
AnnounceDate2023-11-01
AnnouncementXMLSubmission_2023-11-01_06:54:52.198.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDupuyJean-William
SpeciesList scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-01-16 08:16:12ID requested
12023-11-01 06:54:52announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Trypanosoma, Co-immunoprecipitations, flagellar motility, ZMYND12
Contact List
CharlesCoutton
contact affiliationLaboratoire de Génétique Chromosomique, CHU Grenoble Alpes, Grenoble, France. INSERM U1209, CNRS UMR 5309, Institute for Advanced Biosciences, Team Genetics Epigenetics and Therapies of Infertility, University of Grenoble Alpes, Grenoble, France.
contact emailccoutton@chu-grenoble.fr
lab head
DupuyJean-William
contact affiliationPlateforme Proteome Bordeaux
contact emailjean-william.dupuy@u-bordeaux.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
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