Iridovirus is an important viral pathogen affecting large yellow croaker. Megalocytivirus FD2018 with a spanning genome of 112,214 bp double-stranded DNA with a G + C content of 53.53% and 130 predicted genes with ORF sizes of 41 to 1293 amino acids, these virion proteins are predicted to be involved in virion assembly, DNA replication, transcription, nucleotide metabolism and protein modification. Proteomic analysis identified 55 proteins in the cell culture supernatants and purified viral particles.