PXD038984

PXD038984 is an original dataset announced via ProteomeXchange.

Title	Developmental mRNA clearance by PIWI-bound endo-siRNAs in Paramecium
Description	The clearance of untranslated mRNAs by Argonaute proteins is essential for embryonic development in metazoans. However, it is currently unknown whether a similar process exists in unicellular eukaryotes. The ciliate Paramecium tetraurelia harbors a vast array of Argonaute proteins of the Piwi-clade involved in various small RNA pathways, many of which have not yet been investigated. In this work, we investigated the function of a Piwi protein, Ptiwi08, whose expression is limited to a narrow time window during development, concomitant with the start of zygotic transcription. We show that Ptiwi08 acts in an endo-siRNA pathway involved in the clearance of a set of mRNAs at the post-transcriptional level. Based on their features, we hypothesized that these mRNAs may be untranslated. In this dataset, we used mass spectrometry to determine whether any protein products of these mRNAs can be found.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:47:15.615.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manfred Heller
SpeciesList	scientific name: Paramecium tetraurelia; NCBI TaxID: 5888;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-20 20:03:03	ID requested
1	2023-05-10 13:58:17	announced
⏵ 2	2023-11-14 08:47:24	announced	2023-11-14: Updated project metadata.

Solberg T, Mason V, Wang C, Nowacki M, Developmental mRNA clearance by PIWI-bound endo-siRNAs in Paramecium. Cell Rep, 42(3):112213(2023) [pubmed]

submitter keyword: Paramecium tetraurelia, Piwi, mRNA clearance, untranslated mRNA, total protein < 25kDa,siRNAs

Mariusz Nowacki
contact affiliation	Institute of Cell Biology University of Bern
contact email	mariusz.nowacki@unibe.ch
lab head
Manfred Heller
contact affiliation	Proteomics and Mass Spectrometry Core Facility, Department for BioMedical Research (DBMR), University of Bern, Bern, Switzerland
contact email	pmscf@dbmr.unibe.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD038984

PRIDE project URI

• PRIDE
  1. PXD038984
     1. Label: PRIDE project
     2. Name: Developmental mRNA clearance by PIWI-bound endo-siRNAs in Paramecium