PXD038984 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Developmental mRNA clearance by PIWI-bound endo-siRNAs in Paramecium |
Description | The clearance of untranslated mRNAs by Argonaute proteins is essential for embryonic development in metazoans. However, it is currently unknown whether a similar process exists in unicellular eukaryotes. The ciliate Paramecium tetraurelia harbors a vast array of Argonaute proteins of the Piwi-clade involved in various small RNA pathways, many of which have not yet been investigated. In this work, we investigated the function of a Piwi protein, Ptiwi08, whose expression is limited to a narrow time window during development, concomitant with the start of zygotic transcription. We show that Ptiwi08 acts in an endo-siRNA pathway involved in the clearance of a set of mRNAs at the post-transcriptional level. Based on their features, we hypothesized that these mRNAs may be untranslated. In this dataset, we used mass spectrometry to determine whether any protein products of these mRNAs can be found. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:47:15.615.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Manfred Heller |
SpeciesList | scientific name: Paramecium tetraurelia; NCBI TaxID: 5888; |
ModificationList | acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-12-20 20:03:03 | ID requested | |
1 | 2023-05-10 13:58:17 | announced | |
⏵ 2 | 2023-11-14 08:47:24 | announced | 2023-11-14: Updated project metadata. |
Publication List
Solberg T, Mason V, Wang C, Nowacki M, Developmental mRNA clearance by PIWI-bound endo-siRNAs in Paramecium. Cell Rep, 42(3):112213(2023) [pubmed] |
Keyword List
submitter keyword: Paramecium tetraurelia, Piwi, mRNA clearance, untranslated mRNA, total protein < 25kDa,siRNAs |
Contact List
Mariusz Nowacki |
contact affiliation | Institute of Cell Biology University of Bern |
contact email | mariusz.nowacki@unibe.ch |
lab head | |
Manfred Heller |
contact affiliation | Proteomics and Mass Spectrometry Core Facility, Department for BioMedical Research (DBMR), University of Bern, Bern, Switzerland |
contact email | pmscf@dbmr.unibe.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038984
- Label: PRIDE project
- Name: Developmental mRNA clearance by PIWI-bound endo-siRNAs in Paramecium