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PXD038984

PXD038984 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDevelopmental mRNA clearance by PIWI-bound endo-siRNAs in Paramecium
DescriptionThe clearance of untranslated mRNAs by Argonaute proteins is essential for embryonic development in metazoans. However, it is currently unknown whether a similar process exists in unicellular eukaryotes. The ciliate Paramecium tetraurelia harbors a vast array of Argonaute proteins of the Piwi-clade involved in various small RNA pathways, many of which have not yet been investigated. In this work, we investigated the function of a Piwi protein, Ptiwi08, whose expression is limited to a narrow time window during development, concomitant with the start of zygotic transcription. We show that Ptiwi08 acts in an endo-siRNA pathway involved in the clearance of a set of mRNAs at the post-transcriptional level. Based on their features, we hypothesized that these mRNAs may be untranslated. In this dataset, we used mass spectrometry to determine whether any protein products of these mRNAs can be found.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:47:15.615.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterManfred Heller
SpeciesList scientific name: Paramecium tetraurelia; NCBI TaxID: 5888;
ModificationListacetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-12-20 20:03:03ID requested
12023-05-10 13:58:17announced
22023-11-14 08:47:24announced2023-11-14: Updated project metadata.
Publication List
Solberg T, Mason V, Wang C, Nowacki M, Developmental mRNA clearance by PIWI-bound endo-siRNAs in Paramecium. Cell Rep, 42(3):112213(2023) [pubmed]
Keyword List
submitter keyword: Paramecium tetraurelia, Piwi, mRNA clearance, untranslated mRNA, total protein < 25kDa,siRNAs
Contact List
Mariusz Nowacki
contact affiliationInstitute of Cell Biology University of Bern
contact emailmariusz.nowacki@unibe.ch
lab head
Manfred Heller
contact affiliationProteomics and Mass Spectrometry Core Facility, Department for BioMedical Research (DBMR), University of Bern, Bern, Switzerland
contact emailpmscf@dbmr.unibe.ch
dataset submitter
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Dataset FTP location
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