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PXD038786 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAmino acid availability acts as a metabolic rheostat to determine the magnitude of ILC2 responses - SLC7A5 and SLC7A8 KO
DescriptionGroup 2 innate lymphoid cells (ILC2) are functionally poised, tissue-resident lymphocytes that respond rapidly to damage and infection at mucosal barrier sites. ILC2 reside within complex microenvironments where they are subject to cues from both the diet and invading pathogens – including helminths. Emerging evidence suggests ILC2 are acutely sensitive not only to canonical activating signals, but also perturbations in nutrient availability. In the context of helminth infection, we identify amino acid availability as a nutritional cue in regulating ILC2 responses. ILC2 were found to be uniquely pre-primed to import amino acids via the large neutral amino acid transporters Slc7a5 and Slc7a8. Cell-intrinsic deletion of these transporters individually impaired ILC2 expansion, while concurrent loss of both transporters markedly impaired the proliferative and cytokine producing capacity of ILC2. Moreover, amino acid determined the magnitude of ILC2 responses in part via tuning of mTOR. These findings implicate essential amino acids as a metabolic requisite for optimal ILC2 responses within mucosal barrier tissues.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-12-12 23:06:29ID requested
12022-12-22 05:42:54announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: ILC2, immunology
Contact List
Dr Matthew R.Hepworth
contact affiliationLydia Becker Institute of Immunology and Inflammation, University of Manchester, M13 9PL, United Kingdom
contact emailmatthew.hepworth@manchester.ac.uk
lab head
contact affiliationUniversity of Dundee
contact emaila.howden@dundee.ac.uk
dataset submitter
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Dataset FTP location
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