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PXD038658

PXD038658 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTargeted cross-linker delivery for in situ mapping of protein conformations and interactions in mitochondria
DescriptionCurrent methods for intracellular protein analysis mostly require the separation of specific organelles or the change of intracellular environment. However, the functions of these proteins are determined by their native microenvironment as they usually form complexes with ions, nucleic acids, and other proteins. Thus, we have explored a method for in situ crosslinking and mapping of mitochondrial proteins in living cells. Poly(lactic-co-glycolic acid) (PLGA) nanoparticles are functionalized with dihexadecyldimethylammonium bromide (DDAB) to deliver protein crosslinkers into mitochondria. In situ crosslinked proteins are analyzed using mass spectrometry. With this method, we have totally identified a total of 74 pairs of74 pairs of protein-protein interactions that do not exist in the STRING database. Our data on mitochondrial respiratory chain proteins (~93%) are also consistent with the experimental or predicted structural analysis of these proteins. Thus, we have established a promising technology platform for in situ defined protein analysis in cellular organelles under their native microenvironment.
HostingRepositoryPRIDE
AnnounceDate2023-05-26
AnnouncementXMLSubmission_2023-05-26_00:54:49.828.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYuwanChen
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-12-07 12:11:43ID requested
12023-05-26 00:54:50announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: In-cell cross-linking, Mitochondria, Protein conformations and interactions, Nanocarrier delivery,Chemical cross-linking mass spectrometry
Contact List
LihuaZhang
contact affiliationCAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
contact emaillihuazhang@dicp.ac.cn
lab head
YuwanChen
contact affiliationCAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
contact emailzpyuwanchen@163.com
dataset submitter
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Dataset FTP location
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