PXD038572

PXD038572 is an original dataset announced via ProteomeXchange.

Title	EBV latent nuclear antigen EBNA3A modulates IRF3-dependent IFNβ expression
Description	Epstein-Barr virus (EBV) persistently infects over 90% of the human population and is the causative agent of infectious mononucleosis and human cancers. For the establishment of life-long infection, EBV tampers with the induction of type I interferon (IFN I)-dependent antiviral immunity in the host. How various EBV latency genes help orchestrate this crucial strategy is incompletely defined. Here, we reveal the mechanism by which the EBV nuclear antigen 3A (EBNA3A) inhibits IFNβ induction. Using proximity biotinylation we identify the histone acetyltransferase P300, a member of the IFNβ transcription complex, as a binding partner of EBNA3A. Follow-up experiments further demonstrate that EBNA3A also interacts with the activated IFN-inducing transcription factor IRF3 that collaborates with P300 in the nucleus and both events are mediated by the N-terminal domain of EBNA3A. Mechanistic studies reveal that EBNA3A reduces the binding of IRF3 to the IFNβ promotor, thereby hampering downstream IFN I signaling. These results reveal a novel mechanism by which viral immune evasion takes place.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_09:13:33.557.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rayman Tjokrodirijo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-06 02:57:54	ID requested
⏵ 1	2025-05-06 09:13:34	announced

10.1016/j.jbc.2024.107645;

Landman SL, Ressing ME, Gram AM, Tjokrodirijo RTN, van Veelen PA, Neefjes J, Hoeben RC, van der Veen AG, Berlin I, expression. J Biol Chem, 300(9):107645(2024) [pubmed]

submitter keyword: immune evasion, viral protein, IRF3, proximity labeling, IFN I, P300,Herpesvirus, viral immunology

Peter A. van Veelen
contact affiliation	Head Proteomics Group Leiden University Medical Center, Center for Proteomics and Metabolomics, The Netherlands
contact email	P.A.van_Veelen@lumc.nl
lab head
Rayman Tjokrodirijo
contact affiliation	Leiden University Medical Center, Proteomics group
contact email	R.T.N.Tjokrodirijo@lumc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD038572

PRIDE project URI

• PRIDE
  1. PXD038572
     1. Label: PRIDE project
     2. Name: EBV latent nuclear antigen EBNA3A modulates IRF3-dependent IFNβ expression