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PXD038466 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePRM quantification of MCS proteins and viral proteins in HCMV virus microenvironment
DescriptionThe control of host processes which influence cell-cell communication is central to determining the outcome of a virus infection in tissue. Despite this, it remains unclear how cells either in close or distant proximity to an infected cell differentially respond to the primary infection. We established a virus infection microenvironment to resolve molecular features and functional consequences of cell spatial address within this localized niche by proteomic analyses, and identified differential regulations of membrane contact site (MCS) proteins in different cell populations. Here, we conducted targeted mass spectrometry (PRM) analyses for all MCS proteins representing every major organelle-organelle association, and identified elevation of MCS proteins in infected and neighboring cells. To check the contribution of extracellular vesicle (EV) dependent mechanism to the viral proteins detected in the neighboring cells, HCMV protein PRM analyses were conducted with or without EV inhibitors, GW4869 or manumycin A (MA), showing that EVs contribute to the viral protein levels in neighboring population.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterBokai Song
SpeciesList scientific name: Human betaherpesvirus 5; NCBI TaxID: 10359; scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListCarbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4)
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-11-30 12:40:28ID requested
12023-05-16 08:05:06announced
22023-05-22 20:22:00announced: Added PubMed Id
Publication List
Song B, Sheng X, Justice JL, Lum KK, Metzger PJ, Cook KC, Kostas JC, Cristea IM, Intercellular communication within the virus microenvironment affects the susceptibility of cells to secondary viral infections. Sci Adv, 9(19):eadg3433(2023) [pubmed]
Keyword List
submitter keyword: virus microenvironment, HCMV, membrane contact site, PRM
Contact List
Ileana Cristea
contact affiliationPrinceton University
contact emailicristea@princeton.edu
lab head
Bokai Song
contact affiliationPrinceton University
contact emailbokais@princeton.edu
dataset submitter
Full Dataset Link List
Panorama Public dataset URI